Protein_Domain

Part:BBa_K877001:Design

Designed by: David Pohlman, Alie Abele   Group: iGEM12_Missouri_Miners   (2012-10-01)


LPP-OmpA and CtCoh2 Combination


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The unstructuralized amino acid linker of LPP-OmpA in combination with a similar structure naturally occurring in the type 2 cohesin domain allowed the two domains to be combined without compromising functionality. The type cohesin amino acid linker was at the 5' end of the sequence while the LPP-OmpA amino acid linker was at the 3' end, because of this the sac1 restriction site added to the cohesin domain was added to the 5' end of its sequence.


Source

The type 2 cohesin domain of this sequence comes from "Clostridium thermocellum" genome sequence.

References